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Part:BBa_K5152010

Designed by: Paul Lau   Group: iGEM24_HongKong-JSS   (2024-09-26)

5' 20 nt overlapping region for pUC19 PstI site

We incorporated overlapping regions into each insert because we're using the HiFi assembly method for assembling all the insert parts in our project. The pUC19 vector was digested and linearized using PstI and EcoRI restriction enzymes.

We also designed a 20 nt overlapping region at the 3' end for the EcoRI site on the pUC19 vector (BBa_K5152011).

We verified that these regions allow for successful and effective assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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